Journal: Journal of Clinical Medicine

Article Title: Intravenous Infusion of Autologous Mesenchymal Stem Cells Expanded in Auto Serum for Chronic Spinal Cord Injury Patients: A Case Series

doi: 10.3390/jcm13206072

Figure Lengend Snippet: Characteristics of processed MSCs.

Article Snippet: For direct assays, aliquots of MSCs at a concentration of 1 × 10 6 cells/mL were immunolabeled at 4 °C for 30 min with the following antihuman antibodies: fluorescein isothiocyanate (FITC)-conjugated CD34 (Becton Dickinson, San Jose, CA, USA), R Phycoerythrin Cyanine 5.1 (PC5)-conjugated CD45 (Beckman Coulter, Brea, CA, USA), and phycoerythrin (PE)-conjugated CD105 (Becton Dickinson).

Techniques: Concentration Assay, Injection

Journal: International Journal of Molecular Sciences

Article Title: Comparative Analysis of Primary Ovarian Cancer Cells and Established Cell Lines as a New Tool for Studies on Ovarian Cancer Cell Complexity

doi: 10.3390/ijms25105384

Figure Lengend Snippet: Characteristics of immortalized ovarian cancer cells. ( a ) The bar charts show the relative expression of the pluripotency-related markers Oct4 , Sox2 , protooncogenic markers p53 , p21 , c-myc , and CD133 . The left bar chart shows the relative expression for OvCa3 A hTERT. The primary ovarian cancer cells OvCa3 A served as a control. The middle and right bar charts show the relative expression for OvCa7 A hTERT, with primary ovarian cancer cells OvCa7 A used as a control. The data represent mean ± SEM values from three independent experiments performed in duplicates. ( b ) Western blot analysis of CD133 molecule expression in immortalized ovarian cancer cell lines. CD133 and β-Actin protein levels were revealed with different sets of specific antibodies in ovarian cancer cell line extracts obtained from lysing cells with the RIPA buffer. Protein extracts from HEPC-CB.1 cells were used as a positive control. CD133—130 kDa and β-Actin—43 kDa expression. Each time, 50 μg/line of total protein was loaded. ( c ) Western blot analysis of Pax8 molecule expression in immortalized cell lines. Pax8 and β-Actin protein levels were revealed with different sets of specific antibodies in cell extracts obtained from lysing cells with the RIPA buffer. Protein extract from OAW-42 cells was used as a positive control, while protein extract from Daudi cells was used as a negative control. Pax8—48 kDa and β-Actin—43 kDa expression. Each time, 50 μg/line of total protein was loaded. The Western blot was performed in one repetition. ( d ) Densitometry analysis of CD133 and Pax8 was performed by using ImageJ software version 1.54f, and is presented as the rate of CD133 and Pax8 molecules expression to β-actin (used as an internal control). The data represent values from one experiment.

Article Snippet: After trypsinization (IIET, Wroclaw, Poland), the cells were washed with PBS and stained with the fluorochrome-conjugated anti-human PE-conjugated antibodies CD73, CD90, CD105, CD44, CD45, CD133 (BD Biosciences, San Jose, CA, USA), FITC-conjugated CD24, CD34 antibodies (BD Biosciences, San Jose, CA, USA), and isotype controls (BD Biosciences, San Jose, CA, USA) at 4 °C for 30 min.

Techniques: Expressing, Western Blot, Positive Control, Negative Control, Software

Journal: International Journal of Molecular Sciences

Article Title: Comparative Analysis of Primary Ovarian Cancer Cells and Established Cell Lines as a New Tool for Studies on Ovarian Cancer Cell Complexity

doi: 10.3390/ijms25105384

Figure Lengend Snippet: Characteristics of spheroids derived from immortalized ovarian cancer cells. ( a ) Mean diameter of spheroids (μm). The data represent mean ± SEM values from 10 spheroids. **** p < 0.0001 calculated vs. OvCa3 A hTERT or OvCa7 A hTERT. ( b ) Representative images of spheroids. The scale bars represent 100 μm. ( c ) The bar chart shows the percentage (%) of positive cells for selected markers (CD133, CD44). For primary cells, the data represent results from one experiment, while for immortalized cells, the data represent mean ± SEM values from three independent experiments. * p < 0.05 calculated vs. OvCa3 A hTERT.

Article Snippet: After trypsinization (IIET, Wroclaw, Poland), the cells were washed with PBS and stained with the fluorochrome-conjugated anti-human PE-conjugated antibodies CD73, CD90, CD105, CD44, CD45, CD133 (BD Biosciences, San Jose, CA, USA), FITC-conjugated CD24, CD34 antibodies (BD Biosciences, San Jose, CA, USA), and isotype controls (BD Biosciences, San Jose, CA, USA) at 4 °C for 30 min.

Techniques: Derivative Assay